Cytokine expression of THP-1 cells infected with <i>S</i>. Typhimurium ST313 and ST19.

<p>Human THP-1 cells were infected with <i>S</i>. Typhimurium I77 (ST19), D65 (ST313) or left uninfected. <b>(A)</b> Total mRNA was isolated from the cells and expression profiles for different proinflammatory cytokines, IL-1β, IL-8 and TNF-α were analyzed by qRT-PCR at 3 h p.i. <b>(B)</b> IL-1β, IL-8 and TNF-α gene expression at 8 h p.i. (<b>C)</b> IL-8 and TNF-α protein expression analyzed at 3 h p.i. <b>(D)</b> IL-8 and TNF-α protein expression analyzed at 8 h p.i.