The role of IFN-I during a primed Cl13 infection.

<p>A–F) Mice were mock (PBS) primed or ArmΔGPC (2×10⁶,i.v.) primed 12 hours prior to infection with Cl13 (2×10⁶,i.v.). At the indicated times virus titers in serum were determined as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004588#ppat-1004588-g001" target="_blank">Fig. 1</a>. G) Mice were infected with Cl13 (2×10<i>⁶</i> ffu, i.v.) and at 24 hours post-infection serum levels of IFNα were measured by ELISA. H) Ly5.1 C57Bl6/J mice and MAVS/IPS-1^-/- mice were γ-irradiated and 5×10⁶ bone marrow cells from either Ly5.1 and MAVS/IPS-1^-/- were injected i.v. into γ-irradiated mice. Mice were fed antibiotic feed for 5 weeks and 8 weeks post γ-irradiation, mice were primed with ArmΔGPC and infected with Cl13 12 hours later. Serum was taken at the indicated time points and assayed for IFNα by ELISA (left) or plaqued on Vero cells (right) (n = 7/group). All data shown are representative of at least 2 independent experiments.