Expression and purification of the recombinant EBA-140 Region II.

<p>A)Western blotting of baculovirus-infected Sf9 cells at 50 h post-infection with MoAb anti-myc (clone 9E10); MW, protein molecular weight standards; L, cell lysate; S, soluble cell fraction; IS, insoluble cell fraction; CM, culture medium. B) Affinity purification of the recombinant EBA-140 Region II on Ni-NTA resin. SDS–PAGE of proteins in the gel stained by CBB; MW, protein molecular weight standards; L, cell lysate; CM, culture medium; FT, flow- through of resin. C) Western blotting of fractions eluted with 50mM (1,2) and 100mM (3, 4) imidazole, with MoAb anti-myc (clone 9E10); RII, Region II (~ 75 kDa).