MAbs 2c and hu2c restrict cell-to-neuron <b>(A-F)</b> and neuron-to-cell spread of HSV-1 <b>(G-L)</b>.

<p>For the investigation of cell-to-neuron spread, epithelial C127I cells (arrows) were infected with the reporter virus HSV-1(17⁺)Lox-Che. At 14 hpi cells were detached, treated with the indicated antibodies and co-cultured with uninfected DRG sensory neurons (arrowheads) <b>(A-E)</b>. For the investigation of neuron-to-cell transmission, DRG neurons were infected with HSV-1(17⁺)Lox-Che. At 24 hpi, GFP-transfected, uninfected C127I were detached, treated with the indicated antibodies and co-cultured with the infected neurons <b>(G-K)</b>. For both settings, cells were fixed as indicated at 7, 22 and 30 hpi with PHEMO and labeled with anti-β-tubulin-III. Images were acquired with a confocal microscope. For the quantification of the cell-to-neuron <b>(F)</b> or neuron-to-cell transmission <b>(L)</b>, the values of mCherry-fluorescence were determined in a defined area within neurons <b>(F)</b> or GFP-positive C127I cells (G). (A-E and G-K) Scale bar is 10 µm. <b>(F and L)</b>. Error bars show standard error of the mean (SEM) of one representative experiment. Differences between the groups were statistically significant by a nonparametric ANOVA one way test (***<i>P</i> < 0.001).