Large deletions occur with CRISPR-Cas9-mediated genome editing.

<p><b>(a)</b> Three sets of PCR primers (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116484#pone.0116484.t004" target="_blank">Table 4</a>) were used to identify large deletions (>600 nt) across the targeted region. The position of the 5′ end of the primer relative to predicted Cas9 cleavage site for the closest sgRNA is shown in parenthesis. The hash marks indicate regions that are not depicted in this panel. Guide B and D target sites are labeled (B) and (D), respectively. The positions of deleted nt are shown with respect the alleles in which they were identified below the schematic of the gene. <b>(b)</b> The identity of the large 42.6kb deletion from allele 2.6.2 is depicted as the sequence tracing of PCR product of the fused gene demonstrates the NHEJ repair junction. The numbering is based on the GRCm38.p2 reference assembly (mm10) for <i>Mus musculus</i>.