Peak heights (cps) of the ion chromatograms of steroids metabolites in cell culture medium.

<p>Data are presented as Mean ± SD. (**) presents statistical significance of differences between groups at p<0.01. The peak height values are presented after division by 1000 (cps/1000).</p><p>C = steroid metabolites analyzed after 48 h incubation of cell culture in absence of enzyme inhibitors. Apigenin = steroid metabolites analyzed after 48 h of cell culture in the presence of apigenin, the specific inhibitor of 17βHSD. Trilostane = steroid metabolites analyzed after 48 h of incubation of cell culture in the presence of trilostane, the specific inhibitor of 3βHSD. The cell density was approx. 5×10⁶ cells/plate. The experiments were conducted in three independent batches with triplicate repeats for each experiment.</p><p>Peak heights (cps) of the ion chromatograms of steroids metabolites in cell culture medium.