Structurally intact NETs still possess histones and MPO after treatment with excess Mg²⁺ and PTase.

<p>Neutrophil extracellular traps were visualized with (<b>A</b>) Sytox Green staining of DNA and anti-histone primary antibodies. (<b>B</b>) Neutrophil extracellular traps were visualized with DAPI staining of DNA and anti-MPO primary antibodies. NETs were not observed in unactivated neutrophils, but were present in PMA-induced NETs that were treated with either excess 5 mM Mg²⁺ or exogenous PTase. Alexa Fluor 647-conjugated secondary antibodies were used to visualize histone H1 and MPO. Representative immunofluorescence images were merged to show overlap of histone H1 and MPO with structurally intact NETs. Scale bars, 10 µm.