Regulation function of <i>tar</i> fitted to reporter gene data when reconstructing protein concentrations from the reporter gene data for physiologically realistic half-lives and including global physiological effects.

<p>As in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1004028#pcbi.1004028.g010" target="_blank">Fig. 10</a>, but the half-lives have now also been estimated from the data, within a physiologically plausible range. Model predictions are in thick solid and dashed blue lines, <i>tar</i> reporter data are in light blue (thin line and shaded area). Two example fits are shown, namely the best fit for estimated half-lives of FliA and FlgM (solid line, <i>Q</i> = 21.0, (<i>k</i>₀,<i>k</i>₁,<i>n</i>,<i>θ</i>,<i>K</i>) = (0.22,6.6,1.38,6252,47467)) and another example of a high-ranking fit (dashed line). In the case of the best fit, the half-lives of FliA are equal to (60,30,24,30,60) min in the (Δ<i>rpoS</i>, Δ<i>cpxR</i>, Δ<i>csgD</i>-M9, Δ<i>csgD</i>-LB, WT-LB) conditions, respectively, while the half-lives of FlgM are equal to (45,7,24,11,9) min. Confidence intervals for the parameter values are reported in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1004028#pcbi.1004028.s010" target="_blank">Text S10</a>.