Cerberus inhibits breast cancer cell migration.

<p><b><i>(A)</i></b> Cerberus prevents wound closure of Nodal expressing breast cancer cells. MCF-7, Hs578t, BT-549 and MDA-MB-231 cells were plated in Ibidi culture insert dishes. Cells were grown in complete medium to 80% confluence, inserts were removed to create a gap and medium was replaced with complete medium supplemented with 2.5 μg/ml Mitomycin C and 0 nM (left panel), 17.8 nM (middle panel), or 178 nM (right panel) Cerberus. Images were taken at 0 h and 24 h after removing insert. <b><i>(B)</i></b> Wound closure evaluation. Images taken at 0 h (blue) and 24 h (green) were analyzed using Wimasis software (Ibidi) to quantify cell migration. Graphs within a panel correspond to experiments carried out with 0 nM (left), 17.8 nM (middle), or 178 nM (right) Cerberus.