<i>fscn1a</i> is required for development of a subset of NC derivatives.

<p>(A) Lateral views of 3 dpf wt and <i>fscn1a MZ</i> embryos stained for <i>th</i>. sym = sympathetic ganglia, scale bar = 100 μm. Mean area of <i>th+</i> sympathetic ganglia is plotted in graph on right. (n = 25 wt, 24 <i>fscn1a MZ</i>, ****p<0.0001). (B) Representative images of <i>dbh:gfp+</i> sympathetic ganglia in 3 dpf wt and <i>fscn1a MZ</i> embryos. Dorsal views, anterior to the left. Scale bar = 25 μm. (C) Representative images of <i>phox2b:gfp+</i> enteric neurons in 3 dpf wt and <i>fscn1a MZ</i> embryos. Dotted lines outline gut. Scale bar = 100 μm. Number of <i>phox2b:gfp+</i> enteric neurons in 625 μm region of gut is plotted in graph on right (n = 15 wt, 13 <i>fscn1 MZ</i>, *p = 0.03). (D) <i>ngn1:gfp+</i> dorsal root ganglia (drg) in 5 dpf wt and <i>fscn1a MZ</i> embryos. Scale bar = 100 μm. (E) Lateral views showing melanophores in 5 dpf wt and <i>fscn1a MZ</i> embryos.