High density/ stiff collagen matrices decrease E2-induced ERE-luciferase expression in MCF-7 and T47D cells.

<p><b>a)</b> MCF-7 cells stably transfected with an ERE-luciferase construct (MELN) were plated in low density (LD) or high density (HD) collagen and treated ± 1nM E2 for 24h, and luciferase activity determined as described in the Materials and Methods. n = 3, *p<0.05, ***p<0.001. <b>b)</b> T47D cells stably transfected with an ERE-luciferase construct (T47D-KBluc) were treated and harvested as in (a). n = 3, **p<0.01, ***p<0.001. <b>c)</b> Matrix density did not affect ERα expression. Lysates from cells plated in LD or HD collagen were examined for ERα expression. Representative blots shown.