AFFITOPE immunization reduces cerebral amyloid deposition in Tg2576 mice.

<p>Groups of Tg2576 mice (n = 10/group) received 6 monthly injections of KLH/ALUM or AD01-, AD02-conjugate vaccines. Brains were isolated, 8 weeks after the 6^th immunization. Quantification of the relative total brain area covered by amyloid deposits (in % of total tissue analyzed) is based on immuno-fluorescence staining using the Aß specific mAb 3A5. Representative subregions of the cortex (A, B) and dentate gyrus (C, D) of controls (A, C) and AD02- (B, D) immunized mice are shown. E) AD02-vaccination reduces the relative area covered by amyloid deposits compared to controls by 70% (diffuse and dense cored amyloid; p˂0.05). F) AD01 vaccination reduces the relative area covered by amyloid deposits compared to controls by 62% (diffuse and dense cored amyloid; p˂0.05). Box plots in E and F show minimum, 25% percentile, median, 75% percentile and maximum. Asterisks in E+F indicate statistical significant difference (p<0.05); Arrowhead in C indicates unspecific fluorescence from a cerebral vessel. Scale bar: 200µM; pictures taken at 10x magnification