The effect of concentration on the histone chaperone-mediated nucleosome assembly process.

<p>(A) The BM time-trace of a 836-<i>bp</i> DNA molecule in response to the addition of 15 nM Asf1 along with different concentration of histone octamer (i) 7.5 nM. (ii) 15 nM. (iii) 30 nM. (iv) 60 nM. (B) The BM time-trace of a 836-<i>bp</i> DNA molecule in response to the addition of 7.5 nM histone octamer along with different concentration of yAsf1 (i) 15 nM. (ii) 37.5 nM. (iii) 75 nM. The black line indicates the initiation of the reaction. The hatched bar represents the expected BM amplitude for 836-bp DNA molecules in response to the addition of histone to form nucleosomes. (C) The distribution of BM amplitude for 836-<i>bp</i> DNA molecules in response to the addition of histone octamer along with different concentration of Asf1 (i) 15 nM (N = 89). (ii) 37.5 nM (N = 51). (iii) 75 nM (N = 31).