Determination of thresholds.

<p>‘True amastigote’ discrimination thresholds based on their (a) ‘Corrected intensity’, and (b) ‘Spot area’ parameters were defined from the spots populations within both, infected and non-infected control wells (lognormal adjustments, blue lines; lower and upper ‘Corrected intensity’ thresholds (respectively at 48 and 274 a.u.), and the upper ‘Spot area’ cut off (51 px²), green lines). Inset in panel (a) zooms in at the spots distribution in the intermediate ‘Corrected intensity’ region (true amastigotes).