Mortalin interacts with ADP and ATP in a micromolar dissociation constant range.

<p>The mortalin interaction with ADP (<b>A</b>) and ATP (<b>B</b>) in the presence of Mg²⁺ was tested by ITC, suggesting K_Ds values of approximately 2.2 ± 0.1 μmol.L^-1 and 1.1 ± 0.1 μmol.L^-1, respectively. Moreover, the ITC data suggested that the interaction was directed by both enthalpy and entropy. <i>Upper panel</i>: The heat released at each ADP or ATP titration is presented by the negative peaks. The red line represents the baseline. <i>Lower panel</i>: The ΔH_app values were calculated by the integrated area of each ADP or ATP titration peak of the upper panel and plotted against the ADP/mortalin molar ratio. The red line represents the fit obtained by the one-site-binding model provided by the Origin program supplied with the ITC device in both cases. The fitting parameters are shown.