TM domain disassociation is required for cytoplasmic domain dissociation-induced high affinity integrin as measured by soluble ligand binding.

<p>Binding of ligand mimetic mAb PAC-1(A) and fibrinogen (Fbg, B) to B1 through B4 cells in the presence of either EDTA (5mM), Ca²⁺ (5mM), Mn²⁺ (1mM), or Ca²⁺(5mM) with DTT (4mM) as indicated. Error bars represent standard deviation (S.D.) from three independent assays.