Nox1 knockdown improved the differentiation of progenitor cell in the ischemic region after cerebral ischemia.

<p>(a) Representative photomicrograph of BrdU (blue) and DCX (red) double immunostaining showing progenitor cells in the peri-infarct region at 2 weeks after ischemia—reperfusion and BrdU (blue) and NeuN (red) double immunostaining showing matured newborn neurons in the peri-infarct region at 4 weeks after ischemia—reperfusion. Scale bars = 50 μm. (b) Schematic diagrams of the infarct core and the peri-infarct region delineated by Nissl staining at 2 weeks after MCAO—reperfusion. Black: infarct core; black dotted line: peri-infarct region; gray colored box: BrdU, DCX, and NeuN immunostaining observed areas (c) BrdU and DCX double-stained cells were counted in the peri-infarct region after cerebral ischemia. Results are presented as the mean ± SEM, n = 6/6. **p<0.01 vs scbRNA control. ## p<0.01 vs scbRNA MCAO (d) BrdU and NeuN double-stained cells were counted in the peri-infarct region after cerebral ischemia. Results are presented as the mean ± SEM, n = 6/8. ***p<0.001 vs scbRNA control, # p<0.05 vs scbRNA MCAO. scbRNA: scramble shRNA.