MyD88 mediates the first stage of neutrophil recruitment to the lung.

<p>(A-B) The bar graphs show the mean (+SEM) (A) lung and (B) BALF neutrophil numbers 10 h and 36 h p.i. in C57BL/6 (black bars) and MyD88^(−/−) (grey bars) mice challenged with 3 × 10⁷ conidia. Data were pooled from 3 independent experiments and include 11–14 mice per group per time point. (C) The flowplots show the frequencies of CD45.1⁺ MyD88^(+/+) and CD45.2⁺ MyD88^(−/−) neutrophils in the BM, blood, lung and BALF in a representative bone marrow chimeric mouse 10 h p.i. with 3 × 10⁷ conidia. CD45.1⁺CD45.2⁺ recipient mice were lethally irradiated and reconstituted with a 3:1 ratio of CD45.2⁺ MyD88^(−/−) to CD45.1⁺ MyD88^(+/+) BM cells. The plots were gated on CD11b⁺Ly6G⁺ neutrophils. (D) The graph shows the mean ratio (±SEM) of MyD88^(−/−) to WT neutrophils (NFs) in BM, blood, lung and BALF pooled from two experiments with 7 mice.