MiR-203 negatively regulates ZNF217 expression via the ZNF217 3’-UTR.

<p>(A) The putative miR-203 binding sequences in ZNF217 3’-UTR. (B) Luciferase activity assay was performed for the HEK293T cells cotransfected with pmiR-REPORTTM vectors containing WT-ZNF217 3’-UTR or MUT-ZNF217 3’-UTR sequences and miR-203 mimics. Data are presented as normalized fold change in luciferase activity. (C, D) ZNF217 mRNA and protein were determined in SW480 cells transfected with miR-203 mimics or miR-negative control by qRT-PCR and Western blot, respectively. (E) Inverse correlation between ZNF217 mRNA expression and miR-203 levels in CRC tissues was analyzed using Pearson’s correlation analysis.