Early GAL pathway induction establishes a growth advantage during carbon source switch.

<p>Measurements of glucose, galactose, and growth rates for delayed galactose experiment in which 0.1% galactose was added to a set of cultures at different times that had all received 0.1% glucose from time zero. Arrows indicate the time of the galactose stimulus. <b>(A)</b> Glucose concentrations as a function of time for each condition. <b>(B)</b> Galactose concentrations as a function of time for each condition. Lines represent fitted Hill functions. <b>(C)</b> Fractional change in the half-max of the galactose decay curves for each condition relative to the culture that received glucose and galactose simultaneously at the beginning of the experiment (0). <b>(D)</b> Normalized growth rates of cultures that received galactose at 3.1, 4.2, 5.3, and 6.3 h after glucose to that of a culture that received galactose and glucose simultaneously (red line). Un-normalized growth rates for each condition (inset). Error bars represent one s.d. from the mean of two replicates. Data for this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002042#pbio.1002042.s001" target="_blank">S1 Data</a>.