The effect of the p21^cip1 genotype on the expression of p21^cip1.

<p>The graphs plot the amount of p21^cip1 protein expressed per cell (Panel A); the p21^cip1 protein density per nucleus (Panel B) and the percentage of nuclear positive cells (Panel C) of cells transfected with either the common or variant p21^cip1. Only cells that were positive for p21^cip1 protein, as determined by Acumen Cytometry, were included in the analysis. To allow comparison of the cells transfected with the different variants of p21^cip1, the expression of p21^cip1 in each transfected population was normalised to that in the common p21^cip1 transfected population. All results concerning p21^cip1 expression in the common population are therefore displayed as 100%. On the x-axis: com p21 represents cells transfected with common p21^cip1; var p21 represents cells transfected with variant p21^cip1. The y-axis represents the p21^cip1 protein expression per cell (Panel A), the p21^cip1 density per nucleus (Panel B), and the percentage of nuclear positive cells (Panel C) expressed as a percentage of that in the population transfected with common p21^cip1. The top of the bars represent the mean. The error bars represent the SEM. Analysis was carried out from 8 technical replicates of more than 5000 cells each.