Altered PP1 expression, CK2 activity and Ikaros nuclear staining pattern in TB mice.

<p>Western blot analysis of <b>A</b>. PP1 and <b>B</b>. CK2α protein expression in control and TB splenocytes. To control for equal protein loading the blots were reprobed with an antibody specific to β-actin. Representative quantifications of normalized densitometric ratios of each western blot are shown. <b>C</b>. Counts per minute (C.P.M.) of CK2 activity in protein lysates from splenocytes from control and TB mice as assayed by an <i>in vitro</i> CK2 kinase assay. Represented is the mean ± S.E.M. of control (n = 3 compared to TB (n = 3) mice).*p<0.05, **p<0.005; (by two-tailed Student’s t test). <b>D</b>. Immunofluorescence microscopy showing Ikaros expression and subcellular localization in control and TB splenocytes (n = 25 cells). Nuclear DNA appears as blue (DAPI), Ikaros as red (Ikaros panel) and Ikaros and DAPI combined (Merged panel). (Magnification, ×240). Representative results from at least three independent immunofluorescence microscopy experiments.