Analysis of MMP-9 and MPO in supernatants of neutrophil isolated from a single PiZZ patient.

<p>Neutrophils were isolated every day from one augmented PiZZ A1AT deficient-patient (GOLD stage III), and one non-augmented PiZZ A1AT deficient-patient (GOLD stage II). (<b><i>A</i></b>) matrix metalloproteinase 9 (MMP-9) and (<b><i>B</i></b>) myeloperoxidase (MPO) were analysed in the supernatants of 1x10⁶ cells/ ml (4 hours, 5% CO₂, 37°C). Neutrophil-associated A1AT was analysed in augmented (<b><i>C</i></b>) and non-augmented (<b><i>D</i></b>) PiZZ A1AT deficient-patient by Western blot using polyclonal antibody against A1AT.