Sequence variations at the DENV 3’UTR during experimental host adaptation.

<p><b>(A)</b> Deep sequencing of viral populations after 10 and 20 passages (P10 and P20) in mosquito and mammalian cells. The area and color of circles represent the frequency and number of mutations of the viral variants, respectively. A schematic fan dendrogram indicating the distance between the variants for each population is also shown. For nucleotide sequences and frequencies of each viral variant also see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004604#ppat.1004604.s001" target="_blank">S1 Fig</a>. <b>(B)</b> Sequence of cloned amplicons corresponding to the complete 3’UTR. For each P10 population obtained, 20 individual clones were sequenced. Cells lines used for adaptation experiments are indicated (mosquito C6/36, mosquito U4.4, and human A549 cells). Mutations are indicated in red and deletions in grey. A conservation plot is presented at the bottom. The three independent experiments in C6/36 cells are indicated on the left (I, II and III).