Distribution of Müller glia in an eye with two major retinae.

<p>A–D: Bright-field view (A), DAPI staining of the nuclei (B), and double-immunostaining for Müller proteins GS (C) and CRALBP (D) of a serial section of the eye shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116171#pone.0116171.g002" target="_blank">Fig. 2</a> (2-month-old P_RPE65-ngn3 mouse). CRALBP⁺ cells were more or less evenly distributed in regions proximal to the RPE, whereas the distribution became patchy in the centrally located region (boxed). E–G: High (2.5X) magnification of the boxed region in B–D, respectively. Arrow points to a piece of retinal tissue with no detectable GS and CRALBP protein. Scale bars (100 μm) in A also applies to B–D and that in E also applies to F, G.