The percent of PRE transported in the Caco-2 cell monolayer as a function of concentration at 120 min.

<p>The monolayer was washed with transport buffer and then pre-incubated for 20 min at 37°C. After removing the transport buffer, fresh transport buffer containing the test samples was added to the AP chamber (1.5 ml) for the AP-to-BL directional studies or the BL chamber (2.5 ml) for the BL-to-AP directional studies. The sample was removed from the receiver chamber at 120 min and immediately assayed. The <i>’P</i>_app value was calculated. All experiments were carried out in triplicate. The data are means ± SD.