Area of Tissue Dissection for Molecular Experiments.

<p>Schematic drawing illustrates rat transverse midbrain section at the level of the inferior colliculus (<b>IC</b>). It corresponds to Plates 52–56 of the adult rat brain atlas [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117601#pone.0117601.ref031" target="_blank">31</a>]. Rectangle encloses area dissected for the isolation of the tissue used in molecular experiments. It includes laterodorsal tegmental nucleus (<b>LDTg</b>) of the ventrolateral periaqueductal gray (<b>vlPAG</b>) and dorsal raphe (<b>DR</b>). Numbers in the upper right corner illustrate distance from Bregma. <i>Abbreviations</i>: <b>Aq</b>, aqueduct (Sylvius); <b>CnF</b>, cuneiform nucleus; <b>LDTgV</b>, laterodorsal tegmental nucleus, ventral part; <b>PnO</b>, pontine reticular nucleus, oral part.