Treatment with the antioxidant <i>N</i>-Acetyl-L-Cysteine (NAC) or with the p53 inhibitor, Pifithrin-α (PFT), prevents ROS formation in hMp84-overexpressing A375 cells.

<p>At 12 h (<b>A</b>) and 24 h (<b>B</b>) after transfection, ROS formation was evaluated by DCF-based assay in EV-cells and p84 cells, incubated with or without NAC (5 mM) or PFT (1 μM). Results are presented as mean ± S.E. of six (A) or three (B) independent experiments. *<i>P</i><0.05; **<i>P</i><0.01; n.s.: non-significant.