Transient over-expression of Calpain-3 (variant hMp84) impairs cell proliferation and leads to cell detachment in A375 melanoma cells.

<p>At 24, 48 and 72 h after transfection, hMp84 protein (p84) was evaluated by Western blot in empty vector- (EV) and in hMp84-transfected cells (p84), both in still adhering, viable cells (<b>A</b>) and in floating cells (<b>B</b>), separately harvested. A typical Western blot out of three is shown. At the same times after transfection, cell proliferation (total cell number) (<b>C</b>) and cell detachment (% of floating cells on total cells) (<b>D</b>) were evaluated in parental, non-transfected cells (W), in EV-cells and in p84-cells. Results are presented as mean ± S.E. of at least four independent experiments. *<i>P</i><0.05; **<i>P</i><0.01.