Impact of S1P during thrombin-induced endothelial barrier dysfunction.

<p><i>A</i>. Time course of changes in TER of HUVEC monolayers treated with 1 U/ml thrombin or vehicle at the indicated time point, followed by addition of 2 μM S1P or vehicle 20 min. later. The TER tracings are an average for N = 8 electrode wells in each group. <i>B</i>. Protrusion frequency of HUVEC expressing GFP-actin treated with 1 U/ml thrombin, followed by 2 μM S1P 20 min later. *P<0.05 versus the 0 min time point when thrombin was added. †P<0.05 versus the 20 min time point when S1P was added. N = 9 cells studied.