Analysis of the interactions between splenic DCs and CD4⁺ T cells after <i>Pc</i> infection.

<p><b>(A)</b> B6.CD11c-YFP mice were i.p. infected with 1 × 10⁶ mCherry-<i>Pc</i> iRBCs. Spleens were analyzed by CIVM after 2 h and 12 h and in non-infected controls. Speed and displacement of YFP⁺ cells are shown. Black, red and blue dots are from three different experiments. Horizontal lines represent mean values and SEM. <b>(B</b> and <b>C)</b> B6 mice were i.p. infected with 1 × 10⁶ mCherry-<i>Pc</i> iRBCs. Spleens were analyzed after 2 h or 24 h and in non-infected controls. <b>(B)</b> Representative immunofluorescence images (10x magnification) show CD11c⁺ cells and CD4⁺ cells in DAPI-stained tissue sections. <b>(C)</b> Percentages of CD11c pixels colocalized with GFP pixels in the immunofluorescence images are shown (means ± SD). <b>(D</b> and <b>E)</b> B6.CD11c-YFP mice were adoptively transferred with 5 × 10⁶ CFP⁺CD4⁺ T cells and i.p. infected with 1 × 10⁶ mCherry-<i>Pc</i> iRBCs. Spleens were analyzed by CIVM after 24 h and in non-infected controls. <b>(D)</b> Snapshots show the subcapsular RP with CFP⁺CD4⁺ cell tracking. <b>(E)</b> Speed and arrest coefficients of CFP⁺CD4⁺ cells are shown. Black, red and blue dots are from three different experiments. Horizontal lines represent mean values and SEM. In <b>B</b> and <b>D</b>, the scale bars correspond to 100 and 50 µm, respectively. In <b>A</b>, <b>C</b> and <b>E</b>, significant differences (p < 0.05) between the indicated groups are designated by *. In <b>A</b> and <b>E</b>, data were calculated using Imaris software. Data from three experiments (n = 2) are shown. In <b>C</b>, data were calculated from eight images (two images per mouse) using FIJI software. In <b>B</b> and <b>C</b>, one representative experiment out of three (n = 4) is shown. In <b>D</b>, one representative experiment out of three (n = 2) is shown.