Gene expression changes in splenocytes isolated from mice with TAI after Saline and Serp-1 treatments.

<p>(A) Real-time PCR analysis demonstrates significantly increased gene expressions for IL-1β, IL-17, and CD25, in spleen isolates from Saline+PBMC treated GCA^pos TA implanted mice (P ≤ 0.0317, 0.0281, and 0.0354, respectively). fII and IL-1β gene expression in GCA^pos engrafted mice are significantly reduced by Serp-1+ PBMC treatment (P ≤ 0.002; n = 3 for GCA^pos and GCA^neg) (B) In combined data of GCA^pos and GCA^neg engrafted mice, PCR array analyses demonstrate significantly increased expression of thrombotic and thrombolytic proteases, serpins and receptors, inflammatory cell markers and cytokines/chemokines in TAI engrafted mice transfused with PBMCs. Serp-1+PBMC treatment significantly reduced expression when compared to Saline + PBMC. (* P ≤ 0.05, ** P ≤ 0.01; N = 4 for Saline + PBMC, N = 5 for Serp-1 + PBMC).