UBE3C up-regulates β-catenin expression and promotes the nuclear accumulation of β-catenin.

<p>(A) Overexpression of <i>UBE3C</i> in 786-O cells increased β-catenin expression (left). But, knockdown of <i>UBE3C</i> expression in ACHN cells reduced β-catenin expression (middle). β-catenin degradation induced by UBE3C knockdown was substantially attenuated when the cells were treated with epoxomicin (right). (B) Overexpression of UBE3C in 786-O cell did not change β-catenin mRNA expression. Knockdown of UBE3C expression also did not induce β-catenin mRNA levels. β-catenin mRNA was measured using qrtPCR. Means±SD from three independent experiments were presented as relative ratio to the control whose value was taken as 1.0. (C) Immunofluorescence assay revealed that exogenous expression of UBE3C indeed promotes β-catenin translocation to the nucleus in 786-O cells.