<p>(A–D) INS-1 cells were cultured for 14 days. (E and F) under normal-culture-glucose (11.2 mmol/l; white bar) or experimental-high-glucose (22.4 mmol/l; black bar) conditions. (G and H) INS-1 cells cultured in 11.2 mmol/l glucose conditions with palmitate for 14 days. Insulin mRNA levels (A, C, E, and G) were examined by real-time PCR analysis. DNA methylation of the <i>Ins1</i> promoter (B, D, F, and H) was examined by pyrosequencing analysis. (I) INS-1 cells were cultured for 14 days under the indicated conditions. Following this, GSIS was performed with low glucose (2.8 mmol/l; white bar) or high glucose (16.7 mmol/l; black bar) for 30 min. All results are mean ± SEM (<i>n</i> ≥ 4). Asterisks indicate statistically significant differen...
Aims/hypothesis: Although recent studies propose that epigenetic factors influence insulin expressio...
<div><p>The onset of metabolic dysregulation in type 1 diabetes (T1D) occurs after autoimmune destru...
Insulin is a critical component of metabolic control, and as such, insulin gene expression has been ...
<p>INS-1 cells were cultured for 14 days under the following conditions: (A and B) with H<sub>2</sub...
<p>(A) INS-1 cells were cultured under normal-culture-glucose (11.2 mmol/l; white bar) or experiment...
<p>(A) INS-1 cells were cultured in glucose and palmitate for 14 days. (B-D) INS-1 cells were cultur...
<div><p>Recent studies have implicated epigenetics in the pathophysiology of diabetes. Furthermore, ...
<p>(A) A diagram of the three luciferase reporter plasmids used to test the effect of methylation on...
Recent studies have implicated epigenetics in the pathophysiology of diabetes. Furthermore, DNA meth...
Recent studies have implicated epigenetics in the pathophysiology of diabetes. Further-more, DNA met...
Background: Insulin is a critical component of metabolic control, and as such, insulin gene expressi...
BACKGROUND:Insulin is a critical component of metabolic control, and as such, insulin gene expressio...
<div><p>DNA methylation is a mechanism by which cells control gene expression, and cell-specific gen...
DNA methylation is a mechanism by which cells control gene expression, and cell-specific genes often...
AIMS/HYPOTHESIS: Although recent studies propose that epigenetic factors influence insulin expressio...
Aims/hypothesis: Although recent studies propose that epigenetic factors influence insulin expressio...
<div><p>The onset of metabolic dysregulation in type 1 diabetes (T1D) occurs after autoimmune destru...
Insulin is a critical component of metabolic control, and as such, insulin gene expression has been ...
<p>INS-1 cells were cultured for 14 days under the following conditions: (A and B) with H<sub>2</sub...
<p>(A) INS-1 cells were cultured under normal-culture-glucose (11.2 mmol/l; white bar) or experiment...
<p>(A) INS-1 cells were cultured in glucose and palmitate for 14 days. (B-D) INS-1 cells were cultur...
<div><p>Recent studies have implicated epigenetics in the pathophysiology of diabetes. Furthermore, ...
<p>(A) A diagram of the three luciferase reporter plasmids used to test the effect of methylation on...
Recent studies have implicated epigenetics in the pathophysiology of diabetes. Furthermore, DNA meth...
Recent studies have implicated epigenetics in the pathophysiology of diabetes. Further-more, DNA met...
Background: Insulin is a critical component of metabolic control, and as such, insulin gene expressi...
BACKGROUND:Insulin is a critical component of metabolic control, and as such, insulin gene expressio...
<div><p>DNA methylation is a mechanism by which cells control gene expression, and cell-specific gen...
DNA methylation is a mechanism by which cells control gene expression, and cell-specific genes often...
AIMS/HYPOTHESIS: Although recent studies propose that epigenetic factors influence insulin expressio...
Aims/hypothesis: Although recent studies propose that epigenetic factors influence insulin expressio...
<div><p>The onset of metabolic dysregulation in type 1 diabetes (T1D) occurs after autoimmune destru...
Insulin is a critical component of metabolic control, and as such, insulin gene expression has been ...