Analysis of transcriptional changes in cells primed to the stage of hepatoblasts with or without prior DE specification.

<p>hESCs; human ES cells. DE; definitive endoderm. DE D4; cells transitioned through DE stage and harvested at day 4. Prog D4; cells cultured directly in hepatic progenitor medium and harvested at day4. DE D12: cells transitioned through DE and harvested at day 12. Prog D12; cells cultured directly in hepatic progenitor medium and harvested at day 12. The housekeeping gene GAPDH was used for normalization of the qRT-PCR results. uHepG2; internal control for efficiency of hepatoblast formation. qRT-PCR data are mean ± 1SD of 3 biological replicates.