Western blot analysis to screen for candidate antibody-generating proteins of the live HSV-2 0ΔNLS vaccine.

<p>Representative Western blots of (UI) uninfected Vero cells or cells inoculated with 2.5 pfu/cell of HSV-1 KOS or HSV-2 MS incubated with 1:20,000 dilutions of serum from <b>(A)</b> mock-immunized mice (naïve) or mice immunized with <b>(B)</b> gD-2 + alum/MPL adjuvant, <b>(C)</b> HSV-2 0ΔNLS (<i>ICP0</i>^-) virus, or <b>(D)</b> an acyclovir-restrained HSV-2 MS infection (MS+ACV). Red diamonds (1–9) denote the positions of HSV-2 proteins most commonly targeted by mouse IgG antibodies, and the open arrow denotes the MW of gD-2.