Activity assay of Ang.

<p>(A) Indicated amounts of Ang were added to 96-well plates containing 5,000 U87MG cells/well. After incubation for 48h, cell growth was measured by MTT assay. The data represent the means of three independent experiments. B~C. U87MG cells were transfected with shAng or shNC. The cells were lysed 48 h post-transfection and probed with anti-Ang or anti-GAPDH antibodies(B) or analyzed by double staining with Annexin V-FITC/PI and flow cytometry(C). D. The expression of Ang and Bcl-xL were detected in Ang-treated U87MG cells. The cells were incubated with 0, 10, 50, 250, and 1000 ng/ml Ang respectively for 2 h.