Lack of FcRγ or Mincle does not affect the innate immune response to <i>S. pneumoniae</i> in different cell types.

<p>(A) AECs, MVECs and AMΦs were isolated from the lung, and PMNs and BMMs from the bone marrow of C57Bl/6 mice. Cells were left untreated or infected with <i>S. pneumoniae</i> ST2 (D39). (B-G) AMΦs, BMMs and PMNs were isolated from WT, <i>Fcer1g^-/-</i> and <i>Mincle^-/-</i> mice. (B, C) AMΦs or (D) BMMs were infected with <i>S. pneumoniae</i> ST2 or (E) BMMs were stimulated with TDM as a positive control for 16 h and cytokine release was quantified by ELISA. (F) Untreated or LPS-treated (100 ng/ml, 4 h) BMMs were infected with <i>S. pneumoniae</i> ST2Δ<i>cps</i> (MOI = 2.5) and were treated with gentamicin (50 mg/ml) after 30 min. Intracellular, viable bacteria were determined 60 min or 120 min post infection. (G) Rabbit serum-opsonized <i>S. pneumoniae</i> ST2Δ<i>cps</i> were incubated with PMNs and neutrophil-mediated killing was assessed after 45 min incubation. Data are shown as mean + SEM of (A) two (A: PMN, E, F), three (C, G), four (A: AEC, MVEC, B, D) or five (A: AMΦ, BMM) independent experiments carried out in duplicates (A-F) or quadruplicates (G); * = p<0.05; ** = p<0.01; *** = p<0.001; n.s. not significant.