Histone acetyltransferase (HAT) inhibitors and knockdown suppress HCV replication.

<p>HuH-7-HCV-2a J6/JFH-1(p7-rLuc2A) replicon cells were incubated with DMSO or different concentrations of p300i (<b>A</b>) or HATIIi (<b>B</b>) for 48 h. <i>Renilla</i> luciferase activity was measured and normalized to total protein amount. Statistical differences are indicated as * if <i>p</i> ≤ 0.05, *** if <i>p</i> ≤ 0.001, or <i>NS</i> for not significant. <b>(C)</b>. HuH-7-HCV-2a J6/JFH-1(p7-rLuc2A) replicon cells were transfected with non-silencing control or PCAF RNAi. At 24 h after transfection, <i>renilla</i> luciferase activity was measured and normalized to total protein amount. Statistical differences are indicated as * if <i>p</i> ≤ 0.05. <b>(D)</b>. To determine the transcript level of PCAF, RNA was extracted from HuH-7-HCV-2a J6/JFH-1(p7-rLuc2A) replicon cells 16 h after transfecting with non-silencing control or PCAF RNAi. After reverse transcription, real-time PCR experiment was performed using PCAF-specific primers. The transcript level of β-glucuronidase (GUSB) was determined in parallel and used for normalization. Statistical difference is indicated as * if <i>p</i> ≤ 0.05.