Heat-shock induces overexpression of ATP2C1.
- Waed Btadini (690197)
- Ossama K. Abou Hassan (690198)
- Dana Saadeh (690199)
- Ossama Abbas (690200)
- Farah Ballout (690201)
- Abdul-Ghani Kibbi (14592)
- Ghassan Dbaibo (340842)
- Nadine Darwiche (690202)
- Georges Nemer (295984)
- Mazen Kurban (122847)
Publication date
February 2015
DOIAbstract
<p>(A) Real-time PCR effects of heat-shock on HaCaT cells (p value = 0.004), fibroblasts from HH patient (p value = 0.003), and normal fibroblasts (p value = 0.01). (B) Hsp90 variation in expression before and after heat-shock in different cell types. (C) Western blot results; lane 1: non-heat-shocked normal fibroblasts, 2; heat-shocked normal fibroblasts, 3; non-heat-shocked Hailey-Hailey fibroblasts, 4; heat-shocked Hailey-Hailey fibroblasts. (D) ATP2C1 protein quantification (using image J software) based on the densities of the bands over GAPDH obtained through western blotting showing significant increase in normal fibroblasts (* p value = 0.01), and non-significant increase in HH fibroblasts (** p >0.05).</p
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