Spinal GFP⁺ cells of transgenic <i>uts2b</i>-GFP <i>X. laevis</i> tadpoles mainly express ChAT.

<p>Confocal images of combined immuno-labeling of ChAT⁺ (<b>A1</b>) and GFP⁺ (<b>A2</b>) neurons in a whole-mount spinal cord preparation of stage 50 transgenic tadpole. <b>A3</b>. Merged image obtained when ChAT and GFP stainings were superimposed. Dorsal view with rostral up. Only the hemi-cord is shown. The dashed-line on the right side represents the midline. Note that in this preparation, the more ventral structures are closer to the midline. Confocal images of combined immuno-labeling of ChAT⁺ (<b>B1</b>–<b>C1</b>) and GFP⁺ (<b>B2</b>–<b>C2</b>) neurons in lumbar spinal cord (<b>B</b>) and brainstem (<b>C</b>) cross-sections of stage 60 <i>uts2b</i>-GFP tadpoles. <b>B3</b>, <b>C3</b>. Merged images obtained when ChAT and GFP stainings were superimposed. The cross-section in <b>C1</b>–<b>3</b> originates from the caudal hindbrain (rhombomeres 7–8) where IX-XI motor nuclei are located. Yellow arrowheads designate GFP⁺/ChAT⁺ cells; green arrowheads designate GFP⁺/ChAT⁻ cells; White arrowheads designate GFP⁻/ChAT⁺ cells. D, dorsal; M, medial; V4, 4^th ventricule.