D4GDI inhibits growth of <i>M. tb</i> in mice.

<p>Ten wild type C57BL/6 mice were infected with 50–100 CFU of H37Rv by aerosol. Five mice were sacrificed after 24 hr and the bacterial burden in lungs was confirmed to be 50–100 CFU. The remaining five mice were sacrificed after 30 days. Total number of (A) CD4⁺ cells (B) CD4⁺CD25⁺Foxp3⁺ cells (C) CD4⁺CD25⁺Foxp3⁺D4GDI⁺ cells in the lungs of uninfected and <i>M. tb</i>-infected mice were measured by flow cytometry. (D) Twenty C57BL/6 wild type mice were infected with 50–100 CFU of <i>M. tb</i> H37Rv by aerosol. Ten infected mice were given recombinant D4GDI (10ng/ml) through tail vein injection on day 0, 4, 8, 12, 16, 20, 24 and 28 after infection. Thirty days later, bacterial burden in the lungs was measured by plating lung homogenates and counting CFU. Mean values, p values and SEs are shown.