Measurement of DNA damage in ovarian carcinoma cell lines using alkaline comet assay.

<p>A2780/C30 cells (representative of the two OC cell lines) were treated with (A) cisplatin (0–150μg/ml) for 3h or (B) carboplatin (0–300μg/ml) for 24h, and analyzed at the end of the treatment using comet assay. The error bars represent standard deviation. In (C) typical comet assay images of A2780/C30 cells non-treated (NT), treated with 50μg/ml cisplatin (cis-50), 100μg/ml cisplatin (cis-100), 150μg/ml carboplatin (carbo-150) or 300μg/ml carboplatin (carbo-300). All assays were performed in triplicate.