Perostin expression in human limbal epithelial cells (HLECs) at different growth stages.

<p><b>(A).</b> Reverse transcription-quantitative real-time polymerase chain reaction displayed the expression levels (relative fold of mRNA) of periostin by 50% and 70% confluent HLECs compared with 100% confluent cells. <b>(B).</b> Representative images showing periostin, integrin β1, TCF4 and p63 immunofluorescent staining (green) with propidium iodide (PI, red) counterstaining in HLECs at different growth stages (50%, 70% and 100% confluence). <b>(C).</b> Percentages of periostin-, integrin β1-, TCF4- and p63-positive cells in 50%, 70% and 100% confluent cultures. Data shown as mean ± standard deviation, n = 3; *p<.05; **p<.01.