Detection of humoral responses in experimentally exposed pigs.

<p>Follow-up of the anti-Tsal antibody responses in pigs exposed to 2 different tsetse fly biting intensities [two-weekly exposure for 6 weeks to 3 flies (low exposure group, n = 4) or weekly exposure for 7 weeks to 30 flies (high exposure group, n = 5)] in the competitive ELISA using TsalNb-5-HRP (<b>A</b>) and TsalNb-11-HRP (<b>B</b>) as detection reagents. Samples from one non-exposed animal were available as negative controls. The mean endpoint O.D._405nm of controls were 2.315 and 2.240 following detection with respectively TsalNb-5-HRP and TsalNb-11-HRP. Presented data are the mean percentage inhibition relative to the controls with the 95% CI obtained for undiluted plasmas.