Moderation of IFN-γ induced JAK-STAT signaling by Ara-LAM in <i>Leishmania</i> infected macrophages.

<p>(A) BALB/c derived peritoneal macrophages were pre-treated with Ara-LAM for 3 hrs, followed by <i>L</i>. <i>donovani</i> infection for 24hrs. Macrophages were treated with rIFN-γ (20ng/ml) for 45min, followed by cell lysate preparation and Western blot for JAK1, p-JAK1, JAK2, p-JAK2, STAT-1 and p-STAT-1. The blots shown are representative of triplicate experiments. (B) Both the nuclear extracts and the cytosolic extracts of differently treated peritoneal macrophages were prepared followed by Western blot to analyze the nuclear translocation of p-STAT-1 in <i>L</i>. <i>donovani</i> infected macrophages. Blots shown here are from one of three representative experiments.