Reactivity of anti-HmuY antibodies with <i>P</i>. <i>gingivalis</i> HmuY protein or its homologs from <i>P</i>. <i>intermedia</i> and <i>T</i>. <i>forsythia</i>.

<p>ELISA was carried out using antibodies raised against purified HmuY protein (anti-HmuY 1) (A) and epitope 2 (anti-HmuY 1–2) (B). No reactivity was observed for anti-HmuY 1–1 (epitope 1) and anti-HmuY 1–3 (epitope 3) antibodies. Overexpressed and purified HmuY (<i>P</i>. <i>gingivalis</i>), PinA, PinO (<i>P</i>. <i>intermedia</i>) and Tfo (<i>T</i>. <i>forsythia</i>) proteins were used as antigens.