Expression of M1/M2 markers and CD36 expression from WT and TLR4-/- mouse liver macrophages.

<p>IHLs from all groups of mice were purified at 24 weeks and then stained with anti-F4/80, anti-CD206 anti-CD68 Abs. <b>(A)</b> The absolute number of F4/80⁺ cells of phenotype M1 (CD206^-CD68⁺) and M2 (CD206⁺CD68^-) are indicated. <b>(B)</b> The CD36 expression, after gating on F4/80 is showed. <b>(C)</b> Fold increase of percentages of CCL2- and CCL3-producing F4/80⁺ cells in liver of WT and TLR4-/- respect to LFD group. Cells were gated on F4/80 vs. side scatter dot plots. These assays were performed by flow cytometry and shown as mean ± SEM of six mice from one experiment representative of four performed.