Yeast-based transactivation analysis of p53 proteins towards a 10 RE panel.

<p>Results were obtained as indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116177#sec002" target="_blank">Material and Methods</a>. Presented are the averages of relative light units (RLU), defined as the light unit normalized by the optical density at 600nm and after the subtraction of the empty values, <i>i</i>.<i>e</i>. the p53-independent expression of the reporter. Error bars plot the standard deviation of at least four independent replicates. The activity of p53 proteins from the indicated species was measured after 6 hrs incubation at 30°C in media containing two concentrations of galactose (0.008% and 0.064%), that regulates the expression of the p53 transgene. Results obtained with fruit fly p53 and worm p53 are plotted with a different scale due to the low induction of the reporter. The p53 REs used are indicated in the X-axis (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116177#pone.0116177.t003" target="_blank">Table 3</a>).