Validation of differential protein expression for up-regulated candidate genes.

<p>We used ELISA to assess secreted protein levels of (A) CCL20 and (B) IL8 at baseline and following stimulation with flagellin. CCL20 and IL8 was selected because they were transcriptionally up-regulated in both ALI and monolayer AEC cultures in response to flagellin. For each condition, mean value ± standard deviation is shown. One-way ANOVA <i>P</i>-values across conditions: <i>P</i> < 0.005 for CCL20 and <i>P</i> < 0.0001 for IL8.